Composite

Part:BBa_K1960900:Design

Designed by: Rengwei Liu   Group: iGEM16_XMU-China   (2016-09-29)

At first, we wanted to construct two systems which can detect pathogenic bacteria, the one is for Gram-negative bacterium, and the other one is for Gram-positive bacterium. The promoter P2 is related to quorum sensing system of most Gram-positive bacterium, but the sequence on 2016 DNA Distribution Kit Plates is "Inconsistent", so we decided to do a synthesis. We designed the sequence using SnapGene with BBa_B0034, a useful RBS, and added BioBrick Prefix and Suffix, then sent the designed sequence to Sangon Biotech, a company who has cooperated with our lab for many years to have this part synthesized.

K1960900A.jpeg

The sequence synthesized was cloned into plasmid pUC-57. The length of this part with BioBrick is 151bp, so there's no matter with the synthesized part, and the sequencing is all right.